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              <text>Brassica oleracea is a common family of crop plants including Cabbage, Broccoli, Cauliflower, Kale and Brussels sprout. Brassicas are susceptible to a host of pests and disease such as Black rot. Immune signal pathways, consisting of essential phosphorylation events, remain poorly studied and unclear in many crop plants such as Brassica oleracea. &#13;
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To identify the most phosphoproteins, high-throughput mass spectrometry-based phosphoproteomics was used. The research group’s standard protocol involves a single TiO2 enrichment step, but still misses some essential phosphoproteins. To investigate whether increasing the number of enrichments improves the phosphoprotein identification, three enrichment steps were used and over 1000 phosphoproteins from 1370 unique peptides were identified. The 3 phosopho-peptide enrichment steps generally increased the peptide yield by 25% compared to the standard 1 enrichment. The difference in physiochemical properties between samples was caused by a higher abundance of more hydrophilic and highly phosphorylated serine peptides in the 1st enrichment.&#13;
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This observed enhancement in phosphopeptide yield will enable a significant increase in phosphoprotein identification and demonstrate more novel phosphorylation events related to immune pathways. Characterising these mechanisms will provide direct experimental evidence for resistance genes and will play an important role in annotating these genes. This annotation can be used to breed disease resistance crops and increase food security.&#13;
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                <text>Brassica oleracea is a common family of crop plants susceptible to disease. In this work the research groups' Mass Spectrometry-based Phosphoproteomics method was improved by increasing TiO2 enrichment steps. This enabled the research group to identify key phosphoproteins involved in immune signal transduction.&#13;
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The general narrative around populism tends to limit its focus to unemployment, immigration and inequality. Germany provides a suitable case study to test if these factors are significant predictors since its Eastern states continue to give the most support to the far-right populist Alternative für Deutschland (AfD), despite East Germany's low levels of income inequality, unemployment and share of foreigners.&#13;
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To explore the relationship between income inequality and populism, quantitative data is used to regress the share of AfD votes on Bundesland Gini coefficients. Our results are then controlled for year dummies, unemployment, share of foreigners, East/West dummies and complemented by further qualitative research on the historical, political and socio-economic context.&#13;
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